Welcome to OpenAc4C

N4-acetylcytidine (ac⁴C) is an ancient and highly conserved chemical marker found in all domains of life. Recent advancements in sequencing techniques have enabled the functional analysis of ac⁴C acetylation by accurately capturing its locations and levels, shedding light on its significant regulatory potential and emerging role in diseases.

We present here OpenAc4C, the first comprehensive knowledgebase exclusively designed for unraveling the ac⁴C epitranscriptome across diverse species, spanning vertebrates, mammals, insects, fungi, plants, bacterial, archaea, and viruses,encompassing a total of 33 species.

By mining a large array of ac⁴C epitranscriptome datasets with deep learning-based pipelines, OpenAc4C features a collection of 523,953 putative ac⁴C sites identified from four distinct next-generation (NGS)-based techniques, alongside novel insights from Oxford Nanopore direct RNA sequencing (ONT)-based samples. Beyond ac⁴C landscape, a total of 536,986 ac4C-affecting variants were identified in seven species. Among them, 4,766 pathogenic ac4C-SNPs may drive ac⁴C dysregulation with an emphasis on their disease pathogenesis.