We have upgraded m7GHub (Bioinformatics, 2020) to version 2.0, an updated resource for a comprehensive collection of m⁷G modifications in various types of RNAs across multiple species. The four main components presented in the first release have all been expanded: an m7GDB database containing 430,898 putative m⁷G sites identified in 23 species, collected from both widely applied next-generation sequencing (NGS) and the emerging Oxford Nanopore direct RNA sequencing (ONT) techniques; an m7GDiseaseDB hosting 156,206 m⁷G-associated variants (involving addition or removal of an m⁷G site), including 3,238 disease-relevant m⁷G-SNPs that may function through epitranscriptome disturbance; and two enhanced analysis modules to perform interactive analyses on the collections of m⁷G sites (m7GFinder) and functional variants (m7GSNPer). We expect that m7Ghub v2.0 should serve as a valuable centralized resource for studying m⁷G modification.